Journal: Evidence-based Complementary and Alternative Medicine : eCAM

Article Title: The Induction of Apoptosis in A375 Malignant Melanoma Cells by Sutherlandia frutescens

doi: 10.1155/2016/4921067

Figure Lengend Snippet: The effect of S. frutescens extract on the viability of (a) A375 melanoma, (b) Colo-800 melanoma, (c) HDF α fibroblast, and (d) Hek 293 cells after 24, 48, and 72 h of treatment. The viability was assessed using the alamarBlue assay and expressed as percentage relative to the control-treated cells (0 mg/mL). Error bars represent the SEM ( n ≥ 3) and ∗ indicates a significant difference from 0 mg/mL ( P < 0.05).

Article Snippet: The human primary dermal fibroblast cell line (HDF α ) was purchased from ScienCell, CA, USA.

Techniques: Alamar Blue Assay, Control

Journal: Advances in Wound Care

Article Title: Efficacy of Concentrated Surfactant-Based Wound Dressings in Wound Repair and Biofilm Reduction

doi: 10.1089/wound.2017.0782

Figure Lengend Snippet: Microscopic images of HDFa in the direct cellular assay following treatment at 1 day. (a), DMEM (culture medium); (b), under Plurogel; (c), ∼0.2 cm away from Plurogel; (d), under PSSD; (e), ∼0.2 cm away from PSSD; (f), under Triton-100; (g), ∼0.2 cm away from Triton-100. Images taken at × 100 magnification. Scale bar: 50 μm. DMEM, Dulbecco's modified Eagle's medium; HDFa, adult human dermal fibroblasts. To see this illustration in color, the reader is referred to the web version of this article at www.liebertpub.com/wound

Article Snippet: Primary adult human dermal fibroblasts (HDFa) (ATCC; LGC Standards, United Kindgom) were maintained in Dulbecco's modified Eagle's medium (DMEM) GlutaMAXTM (GibcoTM; ThermoFisher Scientific, United Kindgom) supplemented with 10% fetal bovine serum (HyCloneTM, GE Healthcare Life Sciences, United Kindgom) and 100 U/mL penicillin–streptomycin (Life Technologies, Paisley, United Kindgom).

Techniques: Modification

Journal: Advances in Wound Care

Article Title: Efficacy of Concentrated Surfactant-Based Wound Dressings in Wound Repair and Biofilm Reduction

doi: 10.1089/wound.2017.0782

Figure Lengend Snippet: Microscopic images of HDFa in the direct cellular assay following treatment at 7 days. (a), DMEM (culture medium); (b), under Plurogel; (c), ∼0.2 cm away from Plurogel; (d), under PSSD; (e), ∼0.2 cm away from PSSD; (f), under Triton-100; (g), ∼0.2 cm away from Triton-100. Images taken at × 100 magnification. Scale bar: 50 μm. To see this illustration in color, the reader is referred to the web version of this article at www.liebertpub.com/wound

Article Snippet: Primary adult human dermal fibroblasts (HDFa) (ATCC; LGC Standards, United Kindgom) were maintained in Dulbecco's modified Eagle's medium (DMEM) GlutaMAXTM (GibcoTM; ThermoFisher Scientific, United Kindgom) supplemented with 10% fetal bovine serum (HyCloneTM, GE Healthcare Life Sciences, United Kindgom) and 100 U/mL penicillin–streptomycin (Life Technologies, Paisley, United Kindgom).

Techniques:

Journal: Advances in Wound Care

Article Title: Efficacy of Concentrated Surfactant-Based Wound Dressings in Wound Repair and Biofilm Reduction

doi: 10.1089/wound.2017.0782

Figure Lengend Snippet: Percentage viability of HDFa following treatment with Plurogel and PSSD in the direct cytotoxicity assay. *versus DMEM p < 0.05.

Article Snippet: Primary adult human dermal fibroblasts (HDFa) (ATCC; LGC Standards, United Kindgom) were maintained in Dulbecco's modified Eagle's medium (DMEM) GlutaMAXTM (GibcoTM; ThermoFisher Scientific, United Kindgom) supplemented with 10% fetal bovine serum (HyCloneTM, GE Healthcare Life Sciences, United Kindgom) and 100 U/mL penicillin–streptomycin (Life Technologies, Paisley, United Kindgom).

Techniques: Cytotoxicity Assay

Journal: Frontiers in Cellular Neuroscience

Article Title: Reprogramming of adult human dermal fibroblasts to induced dorsal forebrain precursor cells maintains aging signatures

doi: 10.3389/fncel.2023.1003188

Figure Lengend Snippet: Phase contrast images of (A) HDFs and (B) resulting hiDFP colonies from 20/30-, 50- and 70-year-old subjects. Scale = 300 μm. HDF, human dermal fibroblast; hiDFP, human induced dorsal forebrain precursor.

Article Snippet: Nine adult human dermal fibroblast cell lines (HDFs) were obtained from Cell Applications Inc or The NINDS Human Cell and Data Repository.

Techniques:

Journal: Frontiers in Cellular Neuroscience

Article Title: Reprogramming of adult human dermal fibroblasts to induced dorsal forebrain precursor cells maintains aging signatures

doi: 10.3389/fncel.2023.1003188

Figure Lengend Snippet: Reprogrammed hiDFPs maintain aging-associated features. (A) SA-βgal activity assay of HDFs and hiDFPs. No statistical significance was determined by two-way mixed ANOVA. (B) Mitochondrial superoxide levels in HDFs and hiDFPs. Data represent mean ± SEM with n = 3. Simple main effect of reprogramming on mitochondrial superoxide levels determined by two-way mixed ANOVA with * for p < 0.05.

Article Snippet: Nine adult human dermal fibroblast cell lines (HDFs) were obtained from Cell Applications Inc or The NINDS Human Cell and Data Repository.

Techniques: Activity Assay

Journal: Frontiers in Cellular Neuroscience

Article Title: Reprogramming of adult human dermal fibroblasts to induced dorsal forebrain precursor cells maintains aging signatures

doi: 10.3389/fncel.2023.1003188

Figure Lengend Snippet: Reprogrammed hiDFPs exhibit increased amounts of DNA methylation as measured by the production of 5-methylcytosine (5-mC). (A) Amount of methylated DNA in hiPSCs and hiDFPs relative to HDF. Significance was determined by one-way ANOVA with Tukey post-hoc test. (B) Amount of methylated DNA in HDFs and hiDFPs. A simple main effect of reprogramming irrespective of age group was determined by two-way mixed ANOVA. Data represent mean ± SEM with n = 3. * for p < 0.05, ** for p ≤ 0.01.

Article Snippet: Nine adult human dermal fibroblast cell lines (HDFs) were obtained from Cell Applications Inc or The NINDS Human Cell and Data Repository.

Techniques: DNA Methylation Assay, Methylation

Journal: Antioxidants

Article Title: Effect of Pholiota nameko Polysaccharides Inhibiting Methylglyoxal-Induced Glycation Damage In Vitro

doi: 10.3390/antiox10101589

Figure Lengend Snippet: ( a ) PNPs protect Hs68 cells against MG treatment. # Followed by a significant difference with the controls ( p < 0.05); A: significant difference with the same concentration in different samples ( p < 0.05); a and b: significant difference with the same sample at different concentrations ( p < 0.05). ( b ) Effect of PNPs on cell growth rate. The cell growth rate was determined from generations 1 to 10. Experiments were conducted in triplicate (n = 3), and the data are expressed as the mean ± SD. The data for the linear regression equation plotted by each group are as follows: y = − 1.4 x + 417.5 (controls), y = − 3.2 x + 362.8 (MG-induced), y = − 1.9 x + 377 (PNP-40), y = − 1.6 x + 382.1 (PNP-60), and y = − 2 x + 384.7 (PNP-80). # Followed by a significant difference with the controls in the 10th generation ( p < 0.05); * followed by significant difference with the MG-treated group in the 10th generation ( p < 0.05).

Article Snippet: Human dermal fibroblast cell line Hs68 (Obtained from ATCC CRL-1635, Manassas, VA, USA) was cultured in Dulbecco’s modified Eagle’s medium (DMEM, Gibco ® , Grand Island, NY, USA) containing 10% fetal bovine serum (FBS, Gibco ® , Grand Island, NY, USA) and antibiotics (100 U penicillin and 100 U/mL streptomycin, Gibco ® , Grand Island, NY, USA) under 5% CO 2 at 37 °C.

Techniques: Concentration Assay

Journal: Antioxidants

Article Title: Effect of Pholiota nameko Polysaccharides Inhibiting Methylglyoxal-Induced Glycation Damage In Vitro

doi: 10.3390/antiox10101589

Figure Lengend Snippet: ( a ) Effect of MG-induced ROS generation in Hs68 cells. Scale bar: 100 μm. ( b ) Quantitative analysis of MG-induced Hs68 cell ROS production with or without PNPs pretreatment. ROS scavenging effect of PNPs (1.5 mg/mL) on Hs68 cells against MG-induced ROS generation obtained from the Image J analysis. a–e: Significant difference with different groups ( p < 0.05).

Article Snippet: Human dermal fibroblast cell line Hs68 (Obtained from ATCC CRL-1635, Manassas, VA, USA) was cultured in Dulbecco’s modified Eagle’s medium (DMEM, Gibco ® , Grand Island, NY, USA) containing 10% fetal bovine serum (FBS, Gibco ® , Grand Island, NY, USA) and antibiotics (100 U penicillin and 100 U/mL streptomycin, Gibco ® , Grand Island, NY, USA) under 5% CO 2 at 37 °C.

Techniques:

Journal: Mechanisms of ageing and development

Article Title: A new model to investigate UVB-induced cellular senescence and pigmentation in melanocytes

doi: 10.1016/j.mad.2020.111322

Figure Lengend Snippet: UVB induces senescence in human epidermal melanocytes. (A) Representative pictures of control and UVB-irradiated mela-nocytes stained for SA-β-Gal on D9 of the experiment. Scale bar 50 μm. (B) Percentage of SA-β-Gal positive melanocytes. For each group at least 300 cells were analyzed. (C) p21, pp53 (serin 15), p53 and Lamin B1 protein expression of UVB-irradiated and the respective control cells were analyzed by western blot on days 4 and 9 of the experiment. Representative pictures are shown. Lysates from HDFs passage 35 and HDFs treated with 33 μM Cisplatin were used as positive controls. GAPDH was used as a protein loading control. (D-E) – Analysis of relative intensity of the Western blot on days 4 (D) and 9 (E) was performed in ImageJ software. Results are presented as mean values ± SD of at least 3 independent experiments. * < 0.05; ** < 0.01; *** < 0.001 and n.s. no statistical significance.

Article Snippet: Human dermal fibroblasts (HDFs) derived from neonatal foreskin (ATCC, Manassas, USA #SCRC-1042), HEK293-T cells and U2OS cells were cultivated with normal DMEM supplemented with 10 % FCS, 2 % glutamine and 1 % penicillin/streptomycin.

Techniques: Control, Irradiation, Staining, Expressing, Western Blot, Software

Journal: Mechanisms of ageing and development

Article Title: A new model to investigate UVB-induced cellular senescence and pigmentation in melanocytes

doi: 10.1016/j.mad.2020.111322

Figure Lengend Snippet: Presence of UVB-irradiated HDFs induces multinucleation and modulate melanogenesis of melanocytes in a co-culture system. (A) Schematic representation of the experimental setup for co-culture. (B) Analysis of cell surface area was performed in ImageJ. At least 30 cells from three independent experiments were analyzed. (C) Percentage of multinucleated cells was calculated for melanocytes cultivated in the presence of UVB-irradiated or control HDFs on D3. (D) Intracellular melanin content measurement was performed on D3 of co-culture. Bars represent mean values of three independent experiments ± SD. (E) Representative pictures of melanocytes stained by FM. Scale bar 50 μm. Insets display detail of cytoplasmic regions containing melanosomes (F) The FM stained melanocytes were separated into groups according to the number of melanosomes per cell. Results are presented as mean values ± SD of three independent replicates. * < 0.05; ** < 0.01; *** < 0.001 and n.s. no statistical significance.

Article Snippet: Human dermal fibroblasts (HDFs) derived from neonatal foreskin (ATCC, Manassas, USA #SCRC-1042), HEK293-T cells and U2OS cells were cultivated with normal DMEM supplemented with 10 % FCS, 2 % glutamine and 1 % penicillin/streptomycin.

Techniques: Irradiation, Co-Culture Assay, Control, Staining